2611r rrid ab 10856082  (Bioss)

Journal: Scientific Reports

Article Title: The roles of IL-17C in T cell-dependent and -independent inflammatory diseases

doi: 10.1038/s41598-018-34054-x

Figure Lengend Snippet: Generation of Il17c −/− mice. ( a ) IL-17C gene targeting strategy. The region of the Il17c gene containing from exon 1 to exon 3 was replaced with a cassette containing a neomycin resistance gene ( Neo r ), flanked by loxP sequences. ( b ) Expression of Il17c mRNA in various tissues from wild-type (n = 3) and Il17c −/− (n = 3) mice, determined by qPCR. The data show the mean + SEM.

Article Snippet: After washing the wells, goat anti-mouse IL-17C polyclonal Ab (AF2306; R&D Systems; 1.6 μg/ml in 1x Assay Diluent), as a detection Ab, was applied to the wells, followed by incubation at room temperature for 1 hour.

Techniques: Expressing

Journal: Scientific Reports

Article Title: The roles of IL-17C in T cell-dependent and -independent inflammatory diseases

doi: 10.1038/s41598-018-34054-x

Figure Lengend Snippet: Strong resistance of Il17c −/− mice to T cell-independent endotoxin shock. Female mice were injected intraperitoneally with LPS. After LPS injection, survival was monitored, and peritoneal fluids were collected at the indicated points. ( a ) Survival of wild-type (n = 47) and Il17c −/− (n = 46) mice after LPS injection. ***p < 0.001. ( b ) The concentrations of IL-17C, IL-1β, IL-6, IL-17A and TNF in peritoneal lavage fluids from wild-type mice (0 h, n = 5; 3 h, n = 5; 6 h, n = 6) and Il17c −/− mice (0 h, n = 5; 3 h, n = 5; 6 h, n = 6) were measured by ELISA. The data show the mean + SEM. *p < 0.05: wild-type mice vs. Il17c −/− mice. ( c ) Peritoneal F4/80 − and F4/80 + cells were stimulated with LPS for the indicated times. The expression levels of Il17c and Il17re mRNAs in the cells were determined by quantitative PCR. The data show the mean + SEM (n = 3). ( d ) The expression levels of Il17c , Il17re , Il1b , Il6 , Il17a and Tnfa mRNAs in the tissues and peritoneal lavage fluid cells from wild-type mice at 0, 3 and 6 h after LPS injection were determined by quantitative PCR. The data show the mean + SEM (n = 5). *p < 0.05, **p < 0.01 and ***p < 0.001 vs 0 h ( c , d ).

Article Snippet: After washing the wells, goat anti-mouse IL-17C polyclonal Ab (AF2306; R&D Systems; 1.6 μg/ml in 1x Assay Diluent), as a detection Ab, was applied to the wells, followed by incubation at room temperature for 1 hour.

Techniques: Injection, Enzyme-linked Immunosorbent Assay, Expressing, Real-time Polymerase Chain Reaction

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: IL-17C/IL-17RE Augments T Cell Function in Autoimmune Hepatitis.

doi: 10.4049/jimmunol.1600977

Figure Lengend Snippet: FIGURE 3. IL-17C expression is upregulated in autoimmune hepatitis. (A and B) WT mice were intravenously injected with 12 mg/kg Con A. (A) Relative mRNA expression of IL-17C at different time points in liver of Con A–treated WT mice, n = 5 for each group. (B and C) Representative image of liver sections from control and Con A–treated mice (B) or angioma and AIH patients (C) that were stained for IL-17C by immunohistochemistry. Scale bars, 100 mm (left and middle) and 50 mm (right), respectively. (D) Representative images of liver sections from control and Con A–treated mice (upper panel) or angioma and AIH patients (lower panel) that were stained for IL-17RE by immunohistochemistry. Scale bars, 100 mm (left and middle) and 50 mm (right), respectively. (E) IL-17C mRNA expression in different cell types isolated from liver of normal (n = 4) or Con A–treated mice (n = 6) at 8 h post injection. (F) IL-17C mRNA expression in liver of WTand Rag12/2 mice 8 h after Con A injection, n = 4–5 mice per group. (G) IL-17C mRNA expression in mouse primary hepatocytes treated for 4 h with IL-6 (20 ng/ml), IL-4 (20 ng/ml), IL-1a (10 ng/ml), IL-17A (50 ng/ml), IFN-g (20 ng/ml), IL-1b (10 ng/ml), TNF-a (10 ng/ml). Data shown are representative of two independent experiments. *p , 0.05, **p , 0.01, ***p , 0.001.

Article Snippet: Immunohistochemical staining was performed using a rabbit antimouse IL-17C polyclonal Ab (Bioss) or a goat anti-human IL-17C polyclonal Ab (R&D), rabbit anti-mouse/human IL-17RE polyclonal Ab (Biorbyt), and then incubated with a HRP-labeled secondary Ab and followed by 3,39-diaminobenzidine staining.

Techniques: Expressing, Injection, Control, Staining, Immunohistochemistry, Isolation

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: IL-17C/IL-17RE Augments T Cell Function in Autoimmune Hepatitis.

doi: 10.4049/jimmunol.1600977

Figure Lengend Snippet: FIGURE 5. IL-17C is required for T and NK cell activation in Con A–induced hepatitis. WT and IL-17C2/2 mice were injected with 12 mg/kg Con A intravenously, and liver MNCs were isolated 24 h later. (A–F) Activation of liver CD4+

Article Snippet: Immunohistochemical staining was performed using a rabbit antimouse IL-17C polyclonal Ab (Bioss) or a goat anti-human IL-17C polyclonal Ab (R&D), rabbit anti-mouse/human IL-17RE polyclonal Ab (Biorbyt), and then incubated with a HRP-labeled secondary Ab and followed by 3,39-diaminobenzidine staining.

Techniques: Activation Assay, Injection, Isolation

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: IL-17C/IL-17RE Augments T Cell Function in Autoimmune Hepatitis.

doi: 10.4049/jimmunol.1600977

Figure Lengend Snippet: FIGURE 7. IL-17C–dependent liver damage re- quires NK cells. (A) Representative FACS of liver NK cells from WT (n = 7) and IL-17C2/2 (n = 9) mice 24 h after Con A treatment. (B) Real-time RT- PCR analysis of TRAIL mRNA level in sorted liver CD4+T, CD8+T, NK, NKT cells of WT and IL-17C2/2

Article Snippet: Immunohistochemical staining was performed using a rabbit antimouse IL-17C polyclonal Ab (Bioss) or a goat anti-human IL-17C polyclonal Ab (R&D), rabbit anti-mouse/human IL-17RE polyclonal Ab (Biorbyt), and then incubated with a HRP-labeled secondary Ab and followed by 3,39-diaminobenzidine staining.

Techniques: Quantitative RT-PCR